List the procedural steps, from start to finish, that are required for the correct use of an automatic micropipette.

List the procedural steps, from start to finish, that are required for the correct use of an automatic micropipette.



1) check that the pipette is set to dispense at the correct volume
2) depress the knob until the point of initial resistance is reached
3) immerse the tip of the pipette into the solution
4) slowly release the plunger to draw solution into the tip
5) withdraw the tip from the solution, then place the tip on the internal wall of the container the solution should be dispensed into
6) depress the knob to the initial point of resistance for a second time
7) pause, then depress the knob as far as it will travel


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