Describe the experiment used to test the proton-motive force. Draw it.

Describe the experiment used to test the proton-motive force. Draw it.



Answer: Used light sensitive bacteriorhodopsin protein incorporated on one end of a vesicle membrane, ATP synthase on the other. The protein is closed when in the dark. Put a high concentration on H+ ions and ADP + Pi outside the vesicle. Then shine light on the vesicle. They found that the ATP was produced. So the proton motive force is coupled with ATP synthesis.


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